| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Dueringer, Halle M (17)
|
NITWST |
57.72 |
58.87 |
1.15 |
| 2 |
Filipski, Julie A (15)
|
NITWST |
59.14 |
1:00.15 |
1.01 |
| 3 |
Choroser, Beatrice C (15)
|
NITWST |
57.34 |
1:00.86 |
3.52 |
| 4 |
Bubar, Carolyn M (15)
|
NITWST |
59.47 |
1:01.87 |
2.40 |
| 5 |
Polowy, McKayla L (17)
|
NIHHSC |
1:00.58 |
1:03.46 |
2.88 |
| 6 |
Stockschlaeder, Amelia R (16)
|
NIHAMB |
1:02.31 |
1:03.83 |
1.52 |
| 7 |
Ziemba, Sarah J (16)
|
NIHAMB |
1:08.12 |
1:06.14 |
-1.98 |
| 8 |
Juzdowski, Alexa H (15)
|
NIHAMB |
1:09.92 |
1:09.10 |
-0.82 |
| 9 |
Clemens, Megan E (15)
|
NITTAC |
1:08.59 |
1:09.72 |
1.13 |
| 10 |
Bement, Emily A (15)
|
NIHAMB |
1:11.39 |
1:11.56 |
0.17 |
| 11 |
Delpriore, Riley A (17)
|
NIGWST |
1:06.23 |
1:12.69 |
6.46 |
| 12 |
Raymond, Gabriella M (16)
|
NIHAMB |
1:19.91 |
1:26.64 |
6.73 |